Study summary · research use only
Delta sleep-inducing peptide crosses the blood-brain-barrier in dogs: some correlations with protein binding
Plain-language summary
Paraphrased from the published abstract below — not a verdict on whether anything works.
This study in anesthetized dogs examined whether delta sleep-inducing peptide (DSIP) and analogs can cross into cerebrospinal fluid (CSF) from peripheral circulation, using a 100 micrograms/kg IV bolus of DSIP, desTrp1-DSIP, or D-Ala3-DSIP with a peptide-specific antibody. Significant CSF increases were found after IV injection of each compound, with chromatography showing the DSIP-related increase attributable to DSIP and desTrp1-DSIP; radiolabeled 125I-N-Tyr-DSIP in CSF co-eluted with intact labeled peptide. CSF/plasma ratios for DSIP and 125I-N-Tyr-DSIP were higher than for tritiated insulin given IV. DSIP was reported to bind reversibly to a large plasma and CSF molecule, more so in blood than CSF, with reduced binding of D-Ala3-DSIP, and free DSIP was described as the form likely crossing the blood-brain barrier, consistent with but not proof of a specific saturable process.
Abstract
To determine if the nonapeptide delta sleep-inducing peptide (DSIP) and some of its analogs can enter the cerebrospinal fluid (CSF) from the peripheral circulation, dogs anesthetized with sodium pentobarbital were injected with a 100 micrograms/kg bolus of DSIP, desTrp1-DSIP, or D-Ala3-DSIP. Using an antibody that is highly specific for these nonapeptides, we found significant increases in CSF after IV injection of each. Column chromatography of CSF withdrawn after an IV bolus of DSIP showed the increase in immunoreactivity to be due to DSIP and desTrp1-DSIP. Chromatography of radioactivity appearing in the CSF after an IV bolus of 125I-N-Tyr-DSIP showed co-elution with the intact labeled peptide. The CSF/plasma ratios for DSIP and 125I-N-Tyr-DSIP were higher than that for tritiated insulin injected IV. It was also shown that DSIP is reversibly bound to a large molecule in dog plasma and CSF, and that the binding is greater in blood than in CSF. The binding protein demonstrates some specificity for DSIP, binding a smaller percentage of D-Ala3-DSIP, and it appears that it is probably free DSIP that crosses the blood-brain-barrier. Although non-specific crossing cannot be ruled out, the results presented here are also consistent with a specific saturable process for DSIP.
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