Study summary · research use only
Synthesis of delta sleep-inducing peptide (DSIP) and its physiological activity
Plain-language summary
Paraphrased from the published abstract below — not a verdict on whether anything works.
This rabbit study describes classical synthesis of protected nonapeptide delta sleep-inducing peptide (DSIP), purified via TFA treatment and DEAE-Sephadex-A25 chromatography, with the alpha-to-beta transposition of the Asp residue reported as absent, confirmed by electrophoresis at pH 3.8, microcrystalline-cellulose TLC, and HPLC. Physiological activity was assessed via intravenous administration or mesodiencephalic ventricular infusion in rabbits, with synthetic DSIP associated with intensified delta and sigma waves on EEG; no additional delta/sigma enhancement occurred with 10 or 20 times higher doses than 5 microgram/rabbit via ventricular infusion. Six-day consecutive intravenous administration (50 micrograms/kg) showed no obvious sign of adaptation to DSIP. The authors suggested the physiological function of endogenous DSIP differs from that of general sleeping medications.
Abstract
Protected nonapeptide--Delta Sleep-Inducing Peptide (DSIP) formula: see text has been synthesized by classical method. The product has been treated with TFA and purified on DEAE-Sephadex-A25 column, pure free nonapeptide obtained and alpha to beta transposition of Asp-residue found to be absent. It has been assayed by electrophoresis at pH 3.8, microcrystallinecellulose TLC and HPLC. The physiological activities of synthetic DSIP are performed on rabbits by using intravenous administration or mesodiencephalic ventricular infusion. Its function of intensifying delta and sigma waves on rabbit's electroencephalogram (EEG) is evident. There is no concomitant increase of delta- and sigma-enhancing effect following mesodiencephalic ventricular infusion of 10 or 20 times higher than 5 microgram/rabbit doses. Results of 6-day consecutive intravenous administration (50 microgram/kg) indicate that there is no obvious sign of adaptation to DSIP. Results suggest that the physiological function of endogenous sleep-inducing peptide is different from that of general sleeping draught.
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