Study summary · research use only
Acetyl-L-carnitine increases follicle survival and stromal cell density in cultured bovine ovarian tissues
Plain-language summary
Paraphrased from the published abstract below — not a verdict on whether anything works.
In this study using cultured bovine ovarian cortical tissues, the authors evaluated effects of acetyl-L-carnitine on follicle survival, stromal cell density, extracellular matrix, and mRNA expression of NRF2, SOD, CAT, GPX, and PRDX6. Ovarian fragments (3 × 3 × 1 mm) were cultured for 6 days in α-MEM+ alone or with 10, 50, or 100 μM acetyl-L-carnitine at 38.5°C with 5% CO2. Tissues were fixed in 4% paraformaldehyde for 12 h for histology or stored at -80ºC for mRNA analysis. The abstract reports that 100 μM acetyl-L-carnitine increased the percentage of morphologically normal follicles and stromal cell density, while acetyl-L-carnitine did not change collagen percentage or mRNA expression of NRF2, SOD, CAT, PRDX6, or GPX1.
Abstract
This study aimed to evaluate the effects of acetyl-L-carnitine on follicle survival and growth, stromal cell density and extracellular matrix, as well as on the expression of mRNA for nuclear factor erythroid 2-related factor (NRF2), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX) and peroxiredoxin 6 (PRDX6) in cultured bovine ovarian cortical tissues. Ovarian fragments (3 × 3 × 1 mm) were cultured for 6 days in α-MEM+ alone or supplemented with 10, 50 or 100 μM acetyl-L-carnitine at 38.5°C with 5% CO2 in humidified air. Before (non-cultured tissues) and after culture, the ovarian fragments were fixed in 4% paraformaldehyde for 12 h for histological analysis or stored at -80ºC for mRNA expression analysis of NRF2, SOD, CAT, PRDX6 and GPX1. The results showed that 100 μM acetyl-L-carnitine increased the percentages of morphologically normal follicles and stromal cell density in cultured ovarian tissues. On the other hand, acetyl-L-carnitine did not influence the percentage of collagen in ovarian tissue nor the expression of mRNAs for NRF2, SOD, CAT, PRDX6 and GPX1. In conclusion, 100 μM acetyl-L-carnitine increased follicle survival and stromal cell density in cultured bovine ovarian tissues but does not influence collagen fibre distribution or the expression of mRNAs for NRF2, SOD, CAT, PRDX6 and GPX1.
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