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Determining Glutathione Levels in Plants
Plain-language summary
Paraphrased from the published abstract below — not a verdict on whether anything works.
This methods chapter describes assays for determining glutathione levels in plants (not an animal or human study), covering how abiotic stress leads plants to accumulate reactive oxygen species (ROS) that react with cellular lipids, proteins, and DNA, and how enzymatic and nonenzymatic antioxidant pathways, including glutathione, can decrease ROS accumulation directly or through the ascorbate-glutathione cycle or glutathione peroxidases. The chapter states that glutathione content can be determined using HPLC or spectrophotometric assays, and provides detailed spectrophotometric methods for determining total, reduced, and oxidized glutathione.
Abstract
Upon exposure to abiotic stresses, plants tend to accumulate excessive amounts of reactive oxygen species (ROS) that inturn react with cellular lipids, proteins, and DNA. Therefore, decreasing ROS accumulation is indispensible to survive under stress, which is accomplished by inducing enzymatic and nonenzymatic antioxidant defense pathways. Glutathione, particularly reduced glutathione (GSH), represents a principal anitioxidant that could decrease ROS through scavenging them directly or indirectly through ascorbate-glutathione cycle or GSH peroxidases. Glutathione content can be determined using HPLC or spectrophotometric assays. In this chapter, we provided detailed assays to determine total, reduced, and oxidized gluathione using spectrophotometric method.
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