Study summary · research use only
Determination of growth hormone releasing peptides metabolites in human urine after nasal administration of GHRP-1, GHRP-2, GHRP-6, Hexarelin, and Ipamorelin
Plain-language summary
Paraphrased from the published abstract below — not a verdict on whether anything works.
In this study, the authors investigated urinary metabolites of the growth hormone releasing peptides GHRP-1, GHRP-2, GHRP-6, Hexarelin, and Ipamorelin after nasal administration, each given to one volunteer, using nano-liquid chromatography high-resolution mass spectrometry. The abstract reports six metabolites of GHRP-1 (parent not detected; GHRP-1 (2-4) free acid detectable up to 27 h); GHRP-2, GHRP-2 free acid and GHRP-2 (1-3) free acid up to 47 h; GHRP-6 mostly excreted unchanged and detected up to 23 h with metabolites for 12 h; and Hexarelin and Ipamorelin metabolizing intensively, with Hexarelin (1-3) and Ipamorelin (1-4) free acids detected after parent withdrawal. It concludes detection windows depend on individual metabolism and administration route.
Abstract
Growth hormone releasing peptides (GHRPs) stimulate secretion of endogenous growth hormone and are listed on the World Anti-Doping Agency (WADA) Prohibited List. To develop an effective method for GHRPs anti-doping control we have investigated metabolites of GHRP-1, GHRP-2, GHRP-6, Hexarelin, and Ipamorelin in urine after nasal administration. Each compound was administrated to one volunteer. Samples were collected for 2 days after administration, processed by solid-phase extraction on weak cation exchange cartridges and analyzed by means of nano-liquid chromatography - high resolution mass spectrometry. Six metabolites of GHRP-1 were identified. GHRP-1 in the parent form was not detected. GHRP-1 (2-4) free acid was detected in urine up to 27 h. GHRP-2, GHRP-2 free acid and GHRP-2 (1-3) free acid were detected in urine up to 47 h after administration. GHRP-6 was mostly excreted unchanged and detected in urine 23 h after administration, its metabolites were detectable for 12 h only. Hexarelin and Ipamorelin metabolized intensively and were excreted as a set of parent compounds with metabolites. Hexarelin (1-3) free acid and Ipamorelin (1-4) free acid were detected in urine samples after complete withdrawal of parent substances. GHRPs and their most prominent metabolites were included into routine ultra-pressure liquid chromatography-tandem mass spectrometry procedure. The method was fully validated, calibration curves of targeted analytes were obtained and excretion curves of GHRPs and their metabolites were plotted. Our results confirm that the detection window after GHRPs administration depends on individual metabolism, drug preparation form and the way of administration.
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