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Measurement of glutathione reductase activity
Plain-language summary
Paraphrased from the published abstract below — not a verdict on whether anything works.
This methods paper (species not specified) describes a continuous spectrophotometric assay for measuring glutathione reductase activity. It explains that reduced glutathione, a thiol essential to survival of cells in most aerobic organisms, is present intracellularly and provides protection from hydroperoxides and free radicals, and that the assay follows reduction of glutathione disulfide to reduced glutathione by monitoring oxidation of NADPH as a decrease in absorbance at 340 nm.
Abstract
Reduced glutathione, a thiol, is essential to the survival of the cells of most aerobic organisms. It is present intracellularly and provides protection from hydroperoxides and free radicals. This unit describes a continuous spectrophotometric assay for reductase activity: it follows the reduction of glutathione disulfide to reduced glutathione by monitoring the oxidation of NADPH as visualized by a decrease in absorbance at 340 nm.
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