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Monitoring glutathione homeostasis in nanoparticle-treated hepatocytes
Plain-language summary
Paraphrased from the published abstract below — not a verdict on whether anything works.
This methods chapter describes an assay for measuring reduced and oxidized glutathione in human hepatocarcinoma (Hep G2) cells following treatment with nanoparticle formulations. It explains that glutathione, a tripeptide (L-γ-glutamyl-L-cysteinyl-glycine) present at millimolar intracellular concentrations, serves as a primary cellular antioxidant defense against oxidative stress, and that an increased ratio of oxidized to reduced glutathione may indicate oxidative stress, while a decreased overall glutathione pool may indicate altered conjugative metabolism or impaired synthesis. The chapter presents a colorimetric method for detecting reduced and oxidized glutathione in this cell system.
Abstract
This chapter describes a method for the analysis of human hepatocarcinoma cells (Hep G2 cells) for reduced and oxidized glutathione, following treatment with nanoparticle formulations. Glutathione is a tripeptide (L-γ-glutamyl-L-cysteinyl-glycine) present intracellularly in millimolar concentrations and one of the primary cellular antioxidant defenses against oxidative stress. An increase in the relative amount of oxidized to reduced glutathione may be indicative of oxidative stress, while a decrease in the overall glutathione pool may be indicative of conjugative metabolism or impaired synthesis. The method presented in this chapter utilizes a colorimetric method for detection of reduced and oxidized glutathione.
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