Study summary · research use only
Antiepileptic activity of delta sleep-inducing peptide and its analogue in metaphit-provoked seizures in rats
Plain-language summary
Paraphrased from the published abstract below — not a verdict on whether anything works.
In this rat study, the authors tested delta sleep-inducing peptide (DSIP) and its analogue DSIP-12 against metaphit-induced generalized audiogenic seizures in adult male Wistar rats, across four groups (saline, metaphit, metaphit+DSIP, metaphit+DSIP-12), with sound stimulation applied hourly and EEG recorded. They report that metaphit produced hypersynchronous epileptiform activity and increased power spectra 0.5-30 h after treatment, with peak seizure severity 7-12 h after injection, and that DSIP and DSIP-12 significantly increased delta and theta frequency bands and decreased incidence, mean seizure grade, and duration of convulsions (P<0.05 and P<0.01). The authors suggest both peptides as potential antiepileptics in this animal model, with DSIP-12 described as more efficient than DSIP.
Abstract
Previous studies have shown that humoral, endogenous and somnogenic, delta sleep-inducing peptide (DSIP) has influence on insomnia, pain, adaptation to stress, epilepsy, etc. We investigated the potential of DSIP and its analogue DSIP-12 (a nonapeptide with alanine in position 2 of DSIP molecule substituted by beta-alanine) to antagonize metaphit (1-[1(3-isothiocyanatophenyl)-cyclohexyl]piperidine) induced generalized, reflex audiogenic seizures in adult male Wistar albino rats. The rats divided in four groups received (i.p.): saline; metaphit; metaphit+DSIP; and metaphit+DSIP-12, respectively. Metaphit-treated animals displaying seizure in eight previous tests received DSIP or DSIP-12 and afterwards audiogenic stimuli were applied at hourly intervals for the next 30 h. The animals were exposed to sound stimulation 60 min after metaphit administration and further on at hourly intervals. Incidence and severity of seizures were behaviorally analyzed. Selected EEGs and power spectra were recorded and analyzed. Metaphit led to hypersynchronous epileptiform activity (polyspikes and spike-wave complexes) and increased power spectra 0.5-30 h after the treatment. Severity of metaphit seizures increased with time to reach the peak 7-12 h after injection. DSIP and DSIP-12 significantly (*P<0.05 and **P<0.01) increased in delta and theta frequency bands and decreased the incidence, mean seizure grade and duration of metaphit convulsions. The results suggest that DSIP and DSIP-12 may be considered as potential antiepileptics in the animal model, DSIP-12 being more efficient than DSIP.
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