Study summary · research use only
[Changes in aldosterone binding activity of kidney cytosol after stress in rats and the regulation]
Plain-language summary
Paraphrased from the published abstract below — not a verdict on whether anything works.
This rat study examined how aldosterone binding activity in kidney cytosol changed after scald-induced stress, and whether the changes could be modulated by antibodies against TNF alpha and IL-1 beta, alpha-melanocyte-stimulating hormone (alpha-MSH), or the KPV peptide. The authors reported two types of aldosterone binding activity in kidney cytosol, and found that binding capacity was lower in heavily scalded rats than in controls, while low-degree scalding produced no significant difference. Injection of anti-TNF alpha and anti-IL-1 beta antibodies, alpha-MSH, or KPV peptide was reported to prevent this decrease in binding capacity in heavily scalded rats. The authors suggested aldosterone binding activity may be down-regulated after severe scald injury, and that this could be reversed by the tested treatments.
Abstract
To observe the changes in aldosterone binding activity of kidney cytosols after pathological stress in rats and the regulation, binding capacity (Rt) and apparent dissociation constant (Kd) of aldosterone binding activity of kidney cytosols in normal, low-degree or heavy-degree scalded rats were measured by radioligand binding assay using [3H]aldosterone as the ligand. Changes in Rt and Kd of aldosterone binding activity were observed after injection of anti-rat TNF alpha and IL-1 beta antibodies, alpha-melanocyte-stimulating hormone (alpha-MSH) and KPV peptide (Ac-D-Lys-L-Pro-D-Val). The results indicated that there were two types of aldosterone binding activities in kidney cytosol with different Rt and Kd, and the Rt of heavy-degree scalded rats (Rt1: 22.4 +/- 5.4 fmol/mg pro, Rt2: 196.3 +/- 32.5 fmol/mg pro) was lower than that of the control group (Rt1: 41.6 +/- 7.2 fmol/mg pro, Rt2: 317.6 +/- 70.0 fmol/mg pro) (P < 0.01; P < 0.01); while the Rt of low-degree scalded rats (Rt1: 41.4 +/- 5.0 fmol/mg pro, Rt2: 314.8 +/- 45.7 fmol/mg pro) was not significantly different from that of the control group (P > 0.05; P > 0.05). Injection of anti-rat TNF alpha and IL-1 beta antibodies, alpha-MSH and KPV prevented Rt of aldosterone binding activity from decrease in kidney cytosol of rats with heavy-degree scald. These findings suggest that aldosterone binding activity may be down-regulated in heavy-degree scalded rats, but it may be reversed by injection of anti-rat TNF alpha and IL-1 beta antibodies, alpha-MSH and KPV.
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