Study summary · research use only
The 14-Kilodalton Human Growth Hormone Fragment a Potent Inhibitor of Angiogenesis and Tumor Metastasis
Plain-language summary
Paraphrased from the published abstract below — not a verdict on whether anything works.
This study examined the 14-kilodalton human growth hormone (14 kDa hGH) N-terminal fragment using B16-F10 murine melanoma cells and human brain microvascular endothelial (HBME) cells, along with an in vivo mouse tumor model. B16-F10 cells transfected with 14 kDa hGH expression vectors showed reduced proliferation and migration and increased apoptosis in vitro, and in vivo, 14 kDa hGH expression was associated with reduced tumor growth, metastasis, and tumor angiogenesis. In HBME cells, 14 kDa hGH expression was associated with reduced proliferation, migration, and tube formation, and increased apoptosis, effects the authors report were abolished when plasminogen activator inhibitor-1 (PAI-1) was stably downregulated. The authors describe these findings as indicating a role for 14 kDa hGH and PAI-1 in inhibiting angiogenesis and tumor progression in this model.
Abstract
The 14-kilodalton human growth hormone (14 kDa hGH) N-terminal fragment derived from the proteolytic cleavage of its full-length counterpart has been shown to sustain antiangiogenic potentials. This study investigated the antitumoral and antimetastatic effects of 14 kDa hGH on B16-F10 murine melanoma cells. B16-F10 murine melanoma cells transfected with 14 kDa hGH expression vectors showed a significant reduction in cellular proliferation and migration associated with an increase in cell apoptosis in vitro. In vivo, 14 kDa hGH mitigated tumor growth and metastasis of B16-F10 cells and was associated with a significant reduction in tumor angiogenesis. Similarly, 14 kDa hGH expression reduced human brain microvascular endothelial (HBME) cell proliferation, migration, and tube formation abilities and triggered apoptosis in vitro. The antiangiogenic effects of 14 kDa hGH on HBME cells were abolished when we stably downregulated plasminogen activator inhibitor-1 (PAI-1) expression in vitro. In this study, we showed the potential anticancer role of 14 kDa hGH, its ability to inhibit primary tumor growth and metastasis establishment, and the possible involvement of PAI-1 in promoting its antiangiogenic effects. Therefore, these results suggest that the 14 kDa hGH fragment can be used as a therapeutic molecule to inhibit angiogenesis and cancer progression.
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