Study summary · research use only
3D-bioengineered model of human skeletal muscle tissue with phenotypic features of aging for drug testing purposes
Plain-language summary
Paraphrased from the published abstract below — not a verdict on whether anything works.
This in vitro study using human cell-derived tissue describes a 3D-printed, bioengineered human skeletal muscle platform designed to model native muscle structure while measuring force generation and contraction. Differentiation and maturation of myocytes were shown via immunocytochemistry and confocal microscopy, with functionality assessed using electrical stimulation and contraction kinetics analysis. To model aging, the bioengineered muscle was treated with tumor necrosis factor alpha, which was associated with morphological and functional changes. As a proof of concept, the authors evaluated Argireline Amplified peptide, described as a cosmetic ingredient that causes muscle relaxation, in both healthy and aged tissue models. The authors state the platform could be used to assess morphological and functional changes in muscle aging, with potential applications in biomedicine, cosmetics, and bio-hybrid robotics.
Abstract
Three-dimensional engineering of skeletal muscle is becoming increasingly relevant for tissue engineering, disease modeling and bio-hybrid robotics, where flexible, versatile and multidisciplinary approaches for the evaluation of tissue differentiation, functionality and force measurement are required. This works presents a 3D-printed platform of bioengineered human skeletal muscle which can efficiently model the three-dimensional structure of native tissue, while providing information about force generation and contraction profiles. Proper differentiation and maturation of myocytes is demonstrated by the expression of key myo-proteins using immunocytochemistry and analyzed by confocal microscopy, and the functionality assessed via electrical stimulation and analysis of contraction kinetics. To validate the flexibility of this platform for complex tissue modeling, the bioengineered muscle is treated with tumor necrosis factorαto mimic the conditions of aging, which is supported by morphological and functional changes. Moreover, as a proof of concept, the effects of Argireline® Amplified peptide, a cosmetic ingredient that causes muscle relaxation, are evaluated in both healthy and aged tissue models. Therefore, the results demonstrate that this 3D-bioengineered human muscle platform could be used to assess morphological and functional changes in the aging process of muscular tissue with potential applications in biomedicine, cosmetics and bio-hybrid robotics.
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