Study summary · research use only
Surface modification of electrospun fibers with mechano-growth factor for mitigating the foreign-body reaction
Plain-language summary
Paraphrased from the published abstract below — not a verdict on whether anything works.
In this study combining in vitro work (species not specified) with rat subcutaneous implantation and rat tendon adhesion models, the authors modified an electrospun polycaprolactone (PCL) fibrous scaffold with the short peptide mechano-growth factor (MGF) to address foreign-body reaction (FBR) following synthetic polymeric scaffold implantation. In vitro, the authors report that macrophages responded to MGF via endocytosis, and that MGF promoted histone acetylation and upregulated STAT6 expression to direct an anti-inflammatory phenotype transition, findings used to modify a silk fibroin (SF)-coated PCL scaffold via click chemistry. The authors report that the MGF-modified scaffold was associated with transformation of macrophages toward an M2 phenotype in vitro, and in rats, with inhibition of FBR at the subcutaneous site and prevention of tissue adhesion.
Abstract
The implantation of synthetic polymeric scaffolds induced foreign-body reaction (FBR) seriously influence the wound healing and impair functionality recovery. A novel short peptide, mechano-growth factor (MGF), was introduced in this study to modify an electrospun polycaprolactone (PCL) fibrous scaffold to direct the macrophage phenotype transition and mitigate the FBR. In vitro studies discovered the cell signal transduction mechanism of MGF regulates the macrophage polarization via the expression of related genes and proteins. We found that macrophages response the MGF stimuli via endocytosis, then MGF promotes the histone acetylation and upregulates the STAT6 expression to direct an anti-inflammatory phenotype transition. Subsequently, an immunoregulatory electrospun PCL fibrous scaffold was modified by silk fibroin (SF) single-component layer-by-layer assembly, and the SF was decorated with MGF via click chemistry. Macrophages seeded on scaffold to identify the function of MGF modified scaffold in directing macrophage polarization in vitro. Parallelly, rat subcutaneous implantation model and rat tendon adhesion model were performed to detect the immunomodulatory ability of the MGF-modified scaffold in vivo. The results demonstrate that MGF-modified scaffold is beneficial to the transformation of macrophages to M2 phenotype in vitro. More importantly, MGF-functionalized scaffold can inhibit the FBR at the subcutaneous tissue and prevent tissue adhesion.
pepmg summarizes the peer-reviewed literature and links to every source — it sells nothing, ships nothing, and gives no medical, dosing, or human-use guidance. Don't just trust this summary: follow the citation to its source and read it yourself. Research use only.