Study summary · research use only
The efficacy study of the combination of tripeptide-10-citrulline and acetyl hexapeptide-3. A prospective, randomized controlled study
Plain-language summary
Paraphrased from the published abstract below — not a verdict on whether anything works.
In this human randomized controlled trial, twenty-four healthy volunteers were assigned to four groups over 60 days: combined acetyl hexapeptide-3 and tripeptide-10 citrulline (G1), tripeptide-10 citrulline alone (G2), acetyl hexapeptide-3 alone (G3), or neither peptide (G4). Skin microtopography parameters cR2 and cR3 and transepidermal water loss (TEWL) using a skin visioscan and tewameter were measured. The abstract reports several statistically significant differences between specific group pairs in cR2, cR3, and TEWL at 20, 40, and 60 days, including a decrease in TEWL associated with acetyl hexapeptide-3 treatment. The authors describe skin changes associated with acetyl hexapeptide-3 and report findings on tripeptide-10 citrulline, while stating the mechanism by which the two peptides might act together was not clear in this study.
Abstract
Bioactive peptides have beneficial effects on the skin. We investigated to evaluate the effect of acetyl hexapeptide-3 and tripeptide-10 citrulline and the possible synergism between these two peptides. Twenty-four healthy volunteers were randomized to receive combination of acetyl hexapeptide-3 with tripeptide-10 citrulline (Group G1), tripeptide-10 citrulline (Group, G2), acetyl hexapeptide-3 (Group G3), or neither peptide (Group G4) for 60 days. Skin properties evaluated included skin microtopography, parameters cR2 and cR3, and transepidermal water loss (TEWL) using a skin visioscan and a tewameter, respectively. After 20 days, the measurements between G1 and G2 groups (cR2 P=.045, cR3 P=.044), G2 and G3 groups (cR2 P=.017, cR3 P=.017), G3 and G4 groups (CR2 P=.022), and G2 and G4 groups (cR3 P=.028) from baseline were significant. After 60 days, measurements between groups G1 and G3 (cR2 P=.016, cR3 P=.025), groups G2 and G3 (cR2 P=.044, cR3= P=.044), and groups G1 and G4 (cR2 P=.025) were significant. After 20 days, changes in TEWL between groups G1 and G3 (P=.03), groups G2 and G3 (P=.045), and groups G3 and G4 (P=.025) were significant. After 40 days, changes between groups G2 and G3 (P=.028) and groups G3 and G4 (P=.01) from baseline were significant. Our results confirm the antiwrinkle activity of acetyl hexapeptide-3. A significant decrease in TEWL with acetyl hexapeptide-3 treatment is observed. We provided clinical evidence for the antiwrinkle efficacy of tripeptide-10 citrulline and possibly TEWL. The underlying mechanism by which these two peptides can act synergistically was not clear in this study.
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