Study summary · research use only
Effects of IGF-I bioavailability on bovine preantral follicular development in vitro
Plain-language summary
Paraphrased from the published abstract below — not a verdict on whether anything works.
In this in vitro bovine study, the authors examined regulation of IGF-I bioavailability on preantral follicle development. Bovine preantral follicles were cultured for 6 days in serum-free medium with increasing doses of Long R3 (LR3) IGF-I or human recombinant IGF-I (hrIGF-I). Both increased follicle diameter dose-dependently (P < 0.05) and increased estradiol production over control (P < 0.05). However, follicles treated with LR3 IGF-I and the highest hrIGF-I concentration (1000 ng/ml) had smaller oocyte/follicle ratios and increased oocyte degeneration compared with controls or physiological hrIGF-I concentrations (P < 0.05). IGF-binding proteins were detected in cultured follicles: IGFBP-3 mRNA in oocytes and IGFBP-2 immunoreactivity in oocytes and granulosa cells. The authors conclude regulation of IGF-I bioavailability by IGFBPs is needed for coordinated oocyte and follicle development.
Abstract
The aim of this study was to determine the effect of regulation of IGF-I bioavailability on preantral follicle development in vitro. Bovine preantral follicles were cultured for 6 days in serum-free medium with increasing doses of Long R3 (LR3) IGF-I (an analog with low affinity for IGF-binding proteins (IGFBPs)), or human recombinant IGF-I (hrIGF-I). Follicle diameter and estradiol production were measured every second day. On day 6, ratios of oocyte/follicle diameter and oocyte morphology were assessed by histological examination, and IGFBP-2 and -3 were detected by immunocytochemistry and in situ hybridization respectively. Both types of IGF-I increased follicle diameter in a dose-dependent manner (P < 0.05) and increased estradiol production over control levels (P < 0.05). However, follicles treated with LR3 IGF-I and the highest concentration of hrIGF-I (1000 ng/ml) had smaller oocyte/follicle ratios, and increased oocyte degeneration, compared with controls or follicles treated with physiological concentrations of hrIGF-I (P < 0.05). IGFBPs were detected in cultured preantral follicles, indicating a requirement for regulation of IGF bioavailability during the early stages of follicular development. Specifically, IGFBP-3 mRNA was found to be expressed in oocytes, and IGFBP-2 immunoreactivity was detected in oocytes and granulosa cells of cultured follicles. In summary, the regulation of IGF-I bioavailability by IGFBPs is necessary for the co-ordination of oocyte and follicle development in vitro.
pepmg summarizes the peer-reviewed literature and links to every source — it sells nothing, ships nothing, and gives no medical, dosing, or human-use guidance. Don't just trust this summary: follow the citation to its source and read it yourself. Research use only.