Study summary · research use only
Monoclonal antibodies to mechano-growth factor
Plain-language summary
Paraphrased from the published abstract below — not a verdict on whether anything works.
In this study, the authors produced two hybridoma clones secreting monoclonal antibodies against mechano-growth factor (MGF) by cell fusion. Both antibodies were of the G1 subclass, and competition experiments indicated they recognized different antigenic determinants. Antibodies of clone 8B9 recognized a human MGF peptide (amino acids 87 to 111) absent from insulin-like growth factor-1 (IGF-1). Affinity binding constants for full-length MGF and the 87-111 peptide were determined by ELISA. The authors state the pair of antibodies can be used in a sandwich-type assay to quantify MGF.
Abstract
Two hybridoma clones secreting monoclonal antibodies (MAbs) to mechano-growth factor (MGF) have been produced by cell fusion technique. Isotyping of the MAbs revealed that both belong to the G1 subclass. The epitope specificity of the MAbs has been examined in competition experiments. No competition was detected, suggesting that the MAbs obtained recognize different antigenic determinants. MAbs of one clone (8B9) recognize human MGF peptide absent in insulin-like growth factor-1 (IGF-1) and comprising amino acids from 87 to 111. Affinity binding constants with the full-length MGF and 87-111 amino acid peptide have been determined by enzyme-linked immunosorbent assay (ELISA). A pair of monoclonal antibodies obtained can be used in a sandwich-type assay to quantify MGF.
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