Study summary · research use only
Procedure for successful interspecific embryo transfer from mouflon (Ovis gmelini musimon) to Spanish Merino sheep (Ovis aries)
Plain-language summary
Paraphrased from the published abstract below — not a verdict on whether anything works.
In this study, embryos from five anesthetized mouflons (Ovis gmelini musimon) superovulated with FSH-o (Ovagen) were transferred into preselected Spanish Merino sheep (Ovis aries), after evaluating recipient uterine and corpus luteum status by transrectal ultrasonography and plasma progesterone levels. The authors reported complete myorelaxation in four of the five donor mouflons, and that ten embryos were transferred into five selected recipient ewes, resulting in four pregnancies and seven live-born lambs, including three sets of twins. The authors concluded that combining plasma progesterone measurement with ultrasonographic assessment of the corpus luteum provided useful information for screening potential embryo-transfer recipients.
Abstract
Embryos from five anesthetized mouflons (Ovis gmelini musimon), superovulated with FSH-o (Ovagen) were transferred into preselected Spanish Merino sheep (Ovis aries). Myorelaxation was complete in four of five donor mouflons. The status of the uterus of potential recipients was evaluated by transrectal ultrasonography, and those ewes with fluid in the uterine horn were rejected. The corpus luteum in each ewe was assessed ultrasonographically the day before surgery. Plasma progesterone levels and the quality of the corpora lutea were the criteria for selection of recipients. Ten embryos were transferred to the five selected Spanish Merino recipients, resulting in four pregnancies and seven live-born lambs, including three sets of twins. This study shows that determination of plasma progesterone levels combined with ultrasonographic assessment of the corpus luteum provides information useful for screening of potential recipients.
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