Study summary · research use only
Effects of interleukin-1-beta, interleukin-6 and tumor necrosis factor-alpha, alone or in association with hexarelin or galanin, on growth hormone gene expression and growth hormone release from pig pituitary cells
Plain-language summary
Paraphrased from the published abstract below — not a verdict on whether anything works.
This study used pituitary cells from adult pigs treated with IL-1beta, IL-6, or TNF-alpha (1, 10, and 100 ng/ml), alone or combined with galanin or hexarelin (10(-8) M), measuring GH mRNA by RT-PCR and GH secretion by ELISA. IL-1beta (1, 10, and 100 ng/ml) and IL-6 (1 and 10 ng/ml) increased GH output. IL-1beta and TNF-alpha (1 and 10 ng/ml) reduced galanin-induced GH secretion, and IL-6 (10 ng/ml) enhanced the response to both GH releasers. GH gene expression rose only with IL-6 at 1 and 10 ng/ml, alone or combined with galanin and hexarelin. The authors propose cytokines may have a paracrine/autocrine role in pituitary GH regulation.
Abstract
We studied the effects of IL-1beta, IL-6 and TNF-alpha on GH gene expression and secretion with or without galanin and hexarelin. Pituitary cells from adult pigs were treated with IL-1beta, IL-6 or TNF-alpha (1, 10 and 100 ng/ml), alone or in association with galanin or hexarelin (10(-8) M): GH mRNA was measured by RT-PCR and GH secretion by ELISA. IL-1beta (1, 10 and 100 ng/ml) and IL-6 (1 and 10 ng/ml) significantly (p < 0.05) enhanced GH output. IL-1beta and TNF-alpha (1 and 10 ng/ml) reduced (p < 0.05) the galanin-induced GH secretion and IL-6 (10 ng/ml) potentiated the effect of both GH releasers (p < 0.05). GH gene expression was increased only by IL-6 at the concentrations of 1 and 10 ng/ml, either alone or in association with both galanin and hexarelin. We hypothesize that cytokines may play a paracrine/autocrine role in GH regulation in the pituitary independently from the intracellular pathways of the GH secretagogues.
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